goat anti mouse igg podconjugate  (SouthernBiotech)

Journal: Vaccines

Article Title: Generation and Evaluation of a Multi-Epitope Vaccine Against Acinetobacter baumannii , a Nosocomial Bacterial Pathogen

doi: 10.3390/vaccines14030275

Figure Lengend Snippet: AMEV5 vaccination generated rAMEV5-specific antibody-producing B-cells and IL-5 secreting T-cells in the spleen. BALB/c mice ( n = 3 per group) were subcutaneously vaccinated with either PBS + AddaS03 (mock) or rAMEV5 (10 µg) + AddaS03 (AMEV5) on days 0 and 14. Splenocytes were prepared on day 21. ( A ) Representative B-cell ELISpots are shown, and the frequency of antibody secreting cells (ASCs) was calculated for cells that secreted antibodies reacting with PBS, rAMEV5 or hen egg lysozyme (HEL, an unrelated antigen control). Wells coated with goat anti-mouse Ig(H+L) served as positive control. ( B ) Representative T-cell ELISpots are shown, and spot forming units (SFUs) were calculated for cells that secreted IL-5 upon stimulation with PBS, rAMEV5, or HEL. Splenocytes stimulated with α-CD3 were used as a positive control. Data are presented as mean ± SD. * p < 0.05, unpaired t-test compared to mock.

Article Snippet: A peroxidase-labeled goat anti-mouse Ig(H+L) antibody (1:4000 dilution; #1010-05 SouthernBiotech, Birmingham, AL, USA), goat anti-mouse IgG1 (1:5000, #1070-05), and goat anti-mouse IgG2a (1:5000 dilution, #1080-05) served as the secondary antibody in the indirect ELISA assay.

Techniques: Generated, Control, Positive Control